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mppm1f  (Thermo Fisher)


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    Structured Review

    Thermo Fisher mppm1f
    <t>PPM1F</t> knock-out embryos show severe defects, resulting in early abortion of development. A PPM1F ± mice were mated and embryos were isolated and genotyped at 12.5, 13.5 and 14.5 days post coitus. B WCLs of MEFs isolated at day E10.5 from regularly developed (putative PPM1F +/+ and PPM1F ±) or malformed (putative PPM1F-/-) embryos were probed with polyclonal <t>α-mPPM1F</t> antiserum (upper panel) or monoclonal α-tubulin (lower panel). C Genomic DNA was extracted from E10.5 fibroblasts as in ( B ). Genotyping PCR identified WT, heterozygous and homozygous ppm1f KO embryos. D Isolated ppm1f −/− embryo at E10.5 (right picture) is smaller in size compared to a wildtype embryo and shows a stunted forebrain and hemorhages (left picture); malformation of the telencephalon (arrow) and branchial archs (arrowhead) in the ppm1f −/− embryo is indicated; scale bar: 1 mm. D Whole-mount X-gal staining and sagittal sections of representative ppm1f +/+ (left picture) and ppm1f ± embryo (right picture) at E10.5 showing strong β-galactosidase expression in the rostral region of the telencephalon, the neural tube, the liver and lung. Tc: telencephalon; FV: forebrain vesicle; NT: neural tube; NL: neural lumen; BA: branchial arch; H: heart; Li: liver; scale bar: 500 μm. Areas marked with numbered boxes are shown enlarged on the right hand side; scale bar enlargement: 150 µm
    Mppm1f, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mppm1f/pmc12180154-367-220-262?v=Thermo+Fisher
    Average 99 stars, based on 1 article reviews
    mppm1f - by Bioz Stars, 2026-07
    99/100 stars

    Images

    1) Product Images from "The phosphatase PPM1F, a negative regulator of integrin activity, is essential for embryonic development and controls tumor cell invasion"

    Article Title: The phosphatase PPM1F, a negative regulator of integrin activity, is essential for embryonic development and controls tumor cell invasion

    Journal: BMC Biology

    doi: 10.1186/s12915-025-02254-3

    PPM1F knock-out embryos show severe defects, resulting in early abortion of development. A PPM1F ± mice were mated and embryos were isolated and genotyped at 12.5, 13.5 and 14.5 days post coitus. B WCLs of MEFs isolated at day E10.5 from regularly developed (putative PPM1F +/+ and PPM1F ±) or malformed (putative PPM1F-/-) embryos were probed with polyclonal α-mPPM1F antiserum (upper panel) or monoclonal α-tubulin (lower panel). C Genomic DNA was extracted from E10.5 fibroblasts as in ( B ). Genotyping PCR identified WT, heterozygous and homozygous ppm1f KO embryos. D Isolated ppm1f −/− embryo at E10.5 (right picture) is smaller in size compared to a wildtype embryo and shows a stunted forebrain and hemorhages (left picture); malformation of the telencephalon (arrow) and branchial archs (arrowhead) in the ppm1f −/− embryo is indicated; scale bar: 1 mm. D Whole-mount X-gal staining and sagittal sections of representative ppm1f +/+ (left picture) and ppm1f ± embryo (right picture) at E10.5 showing strong β-galactosidase expression in the rostral region of the telencephalon, the neural tube, the liver and lung. Tc: telencephalon; FV: forebrain vesicle; NT: neural tube; NL: neural lumen; BA: branchial arch; H: heart; Li: liver; scale bar: 500 μm. Areas marked with numbered boxes are shown enlarged on the right hand side; scale bar enlargement: 150 µm
    Figure Legend Snippet: PPM1F knock-out embryos show severe defects, resulting in early abortion of development. A PPM1F ± mice were mated and embryos were isolated and genotyped at 12.5, 13.5 and 14.5 days post coitus. B WCLs of MEFs isolated at day E10.5 from regularly developed (putative PPM1F +/+ and PPM1F ±) or malformed (putative PPM1F-/-) embryos were probed with polyclonal α-mPPM1F antiserum (upper panel) or monoclonal α-tubulin (lower panel). C Genomic DNA was extracted from E10.5 fibroblasts as in ( B ). Genotyping PCR identified WT, heterozygous and homozygous ppm1f KO embryos. D Isolated ppm1f −/− embryo at E10.5 (right picture) is smaller in size compared to a wildtype embryo and shows a stunted forebrain and hemorhages (left picture); malformation of the telencephalon (arrow) and branchial archs (arrowhead) in the ppm1f −/− embryo is indicated; scale bar: 1 mm. D Whole-mount X-gal staining and sagittal sections of representative ppm1f +/+ (left picture) and ppm1f ± embryo (right picture) at E10.5 showing strong β-galactosidase expression in the rostral region of the telencephalon, the neural tube, the liver and lung. Tc: telencephalon; FV: forebrain vesicle; NT: neural tube; NL: neural lumen; BA: branchial arch; H: heart; Li: liver; scale bar: 500 μm. Areas marked with numbered boxes are shown enlarged on the right hand side; scale bar enlargement: 150 µm

    Techniques Used: Knock-Out, Isolation, Staining, Expressing



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    mppm1f  (Thermo Fisher)
    99
    Thermo Fisher mppm1f
    <t>PPM1F</t> knock-out embryos show severe defects, resulting in early abortion of development. A PPM1F ± mice were mated and embryos were isolated and genotyped at 12.5, 13.5 and 14.5 days post coitus. B WCLs of MEFs isolated at day E10.5 from regularly developed (putative PPM1F +/+ and PPM1F ±) or malformed (putative PPM1F-/-) embryos were probed with polyclonal <t>α-mPPM1F</t> antiserum (upper panel) or monoclonal α-tubulin (lower panel). C Genomic DNA was extracted from E10.5 fibroblasts as in ( B ). Genotyping PCR identified WT, heterozygous and homozygous ppm1f KO embryos. D Isolated ppm1f −/− embryo at E10.5 (right picture) is smaller in size compared to a wildtype embryo and shows a stunted forebrain and hemorhages (left picture); malformation of the telencephalon (arrow) and branchial archs (arrowhead) in the ppm1f −/− embryo is indicated; scale bar: 1 mm. D Whole-mount X-gal staining and sagittal sections of representative ppm1f +/+ (left picture) and ppm1f ± embryo (right picture) at E10.5 showing strong β-galactosidase expression in the rostral region of the telencephalon, the neural tube, the liver and lung. Tc: telencephalon; FV: forebrain vesicle; NT: neural tube; NL: neural lumen; BA: branchial arch; H: heart; Li: liver; scale bar: 500 μm. Areas marked with numbered boxes are shown enlarged on the right hand side; scale bar enlargement: 150 µm
    Mppm1f, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mppm1f/pmc12180154-367-220-262?v=Thermo+Fisher
    Average 99 stars, based on 1 article reviews
    mppm1f - by Bioz Stars, 2026-07
    99/100 stars
    		  Buy from Supplier

    Image Search Results


    PPM1F knock-out embryos show severe defects, resulting in early abortion of development. A PPM1F ± mice were mated and embryos were isolated and genotyped at 12.5, 13.5 and 14.5 days post coitus. B WCLs of MEFs isolated at day E10.5 from regularly developed (putative PPM1F +/+ and PPM1F ±) or malformed (putative PPM1F-/-) embryos were probed with polyclonal α-mPPM1F antiserum (upper panel) or monoclonal α-tubulin (lower panel). C Genomic DNA was extracted from E10.5 fibroblasts as in ( B ). Genotyping PCR identified WT, heterozygous and homozygous ppm1f KO embryos. D Isolated ppm1f −/− embryo at E10.5 (right picture) is smaller in size compared to a wildtype embryo and shows a stunted forebrain and hemorhages (left picture); malformation of the telencephalon (arrow) and branchial archs (arrowhead) in the ppm1f −/− embryo is indicated; scale bar: 1 mm. D Whole-mount X-gal staining and sagittal sections of representative ppm1f +/+ (left picture) and ppm1f ± embryo (right picture) at E10.5 showing strong β-galactosidase expression in the rostral region of the telencephalon, the neural tube, the liver and lung. Tc: telencephalon; FV: forebrain vesicle; NT: neural tube; NL: neural lumen; BA: branchial arch; H: heart; Li: liver; scale bar: 500 μm. Areas marked with numbered boxes are shown enlarged on the right hand side; scale bar enlargement: 150 µm

    Journal: BMC Biology

    Article Title: The phosphatase PPM1F, a negative regulator of integrin activity, is essential for embryonic development and controls tumor cell invasion

    doi: 10.1186/s12915-025-02254-3

    Figure Lengend Snippet: PPM1F knock-out embryos show severe defects, resulting in early abortion of development. A PPM1F ± mice were mated and embryos were isolated and genotyped at 12.5, 13.5 and 14.5 days post coitus. B WCLs of MEFs isolated at day E10.5 from regularly developed (putative PPM1F +/+ and PPM1F ±) or malformed (putative PPM1F-/-) embryos were probed with polyclonal α-mPPM1F antiserum (upper panel) or monoclonal α-tubulin (lower panel). C Genomic DNA was extracted from E10.5 fibroblasts as in ( B ). Genotyping PCR identified WT, heterozygous and homozygous ppm1f KO embryos. D Isolated ppm1f −/− embryo at E10.5 (right picture) is smaller in size compared to a wildtype embryo and shows a stunted forebrain and hemorhages (left picture); malformation of the telencephalon (arrow) and branchial archs (arrowhead) in the ppm1f −/− embryo is indicated; scale bar: 1 mm. D Whole-mount X-gal staining and sagittal sections of representative ppm1f +/+ (left picture) and ppm1f ± embryo (right picture) at E10.5 showing strong β-galactosidase expression in the rostral region of the telencephalon, the neural tube, the liver and lung. Tc: telencephalon; FV: forebrain vesicle; NT: neural tube; NL: neural lumen; BA: branchial arch; H: heart; Li: liver; scale bar: 500 μm. Areas marked with numbered boxes are shown enlarged on the right hand side; scale bar enlargement: 150 µm

    Article Snippet: The following antibodies were used with the corresponding dilutions for western blot analysis (WB), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), or integrin activity assay (IA): α-Actinin (BM75.2, mouse anti-human, Abcam; 1:1000 WB), α 1 -integrin (TS2/7, mouse anti-human/anti-mouse, Abcam; 1:50 IF), α 2 -integrin (6 F1, mouse anti-human/anti-mouse, DSHB; 1:60 IF), α 3 -integrin (P1B5, mouse anti-human/anti-mouse, DSHB; 1:60 IF), α 5 -integrin (BIIG2, rat anti-human/anti-mouse, DSHB; 1:10 IF), α v -integrin (PE-P2 W7 mouse anti-human/anti-mouse, sc-9969; IF 1:300), β 1 -integrin (HMβ1-1, armenian hamster anti-mouse, Bio Legend; 1:300 IF; AIIB2, rat anti-human/anti-mouse, DSHB; 1:600 IF, IA; M-106, rabbit anti-mouse/anti-human, Santa Cruz; 1:500 WB; D2E5, rabbit anti-human, Cell Signaling; 1:1000 WB), human β 1 -integrin (P5D2, mouse anti-human, DSHB, 2.5 μg IP; 9EG7, rat anti- human, DSHB 2.5 μg IP; AIIB2, rat anti-human, DSHB; 2.5 μg IP), β 3 -integrin (2 C9.G3, arm. hamster anti-mouse, eBioscience; 1:300 IF; PM6/13, mouse anti-human, Abcam; 1:100 IF), β 5 -integrin (KN-52, mouse anti-mouse/human, eBioscience; IF 1:300), Focal adhesion kinase (FAK) (77, mouse anti-human, BD; 1:250 WB), integrin-linked kinase (ILK) (EP1593Y, rabbit anti-human, Epitomics; 1:800 WB), Kindlin-2 (3 A3, mouse anti-human, Millipore; 1:200 WB, 1:250 IF), Laminin (ab11575, rabbit anti-mouse, Abcam; 1:300 IHC), Nestin (rat-401, anti-mouse, Millipore; IHC 1:200), Paxillin (5H11, mouse monoclonal, Thermo Scientific; 1:1000 WB), hPPM1F (17,020–1-AP, rabbit anti-human, Protein-Tech; 1:1000 WB), mPPM1F (#1147, rabbit anti-mouse PPM1F; generated at the central animal care facility; University of Konstanz; 1:200 WB; see Additional File2: Fig. S2), FilaminA (EP2405Y, IgG, rabbit anti-human, Epitomics; 1:125.000 WB), Tubulin (E7, IgG1, mouse anti-human, DSHB; 1:1000), Talin (8 d4, mouse anti-human, Thermo Scientific; 1:800 WB, 1:40 IF), Vinculin (hVIN-1, mouse anti-human, Sigma; 1:2000 WB, 1:200 IF), Zyxin (Zol301, mouse anti-human, Abcam; 1:1000 WB), Dylight488-conjugated goat anti-mouse IgG (Jackson; 1:200), Cy3-conjugated goat anti-rabbit IgG (Jackson; 1:200), Cy3-conjugated goat anti-mouse IgG (Jackson; 1:200), Cy5-conjugated goat anti-mouse IgG (Jackson; 1:200), RhodamineRed-conjugated goat anti-rat IgG (Jackson; 1:200), RhodamineRed-conjugated goat anti-Armenian Hamster IgG (Jackson; 1:200), HRP-conjugated goat anti-mouse IgG (Jackson; WB 1:10 000), HRP-conjugated goat anti-rat IgG (Santa Cruz; 1:250), HRP-conjugated goat anti-rabbit IgG (Jackson; WB 1:3000), unspecific control IgG (anti-mouse, 96/1, generated at the Tierforschungsanlage; University of Konstanz; anti-rat, MJ7/18 Endoglin, DSHB).

    Techniques: Knock-Out, Isolation, Staining, Expressing